The Apostles and other disciples at the Upper Room were the first to be born again.
1 When the Day of Pentecost had fully come, they were all with one accord in one place.
2 And suddenly there came a sound from heaven, as of a rushing mighty wind, and it filled the whole house where they were sitting.
3 Then there appeared to them divided tongues, as of fire, and one sat upon each of them.
4 And they were all filled with the Holy Spirit and began to speak with other tongues, as the Spirit gave them utterance. (NKJV)